





| Numéro de catalogue | HF879107 |
|---|---|
| Reactivité des espèces | Human |
| Applications | ELISA, FCM |
| Espèce hôte | Mouse |
| Isotype | IgG2a, kappa |
| ID de clone | SAA0415 |
| Clonalité | Monoclonal |
| Cible | TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1 |
| Niveau d'endotoxines | Please contact with the lab for this information. |
| Pureté | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Numéro d'accès | P01375 |
| Forme | Liquid |
| Tampon de stockage | 0.01M PBS, pH 7.4, 0.09% Sodium azide. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |

Flow-cytometry using anti-human TNFa antibody.TNFa Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa antibody monoclonal antibody (Catalog # HF879107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human TNFa antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa antibody monoclonal antibody (Catalog # HF879107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog: HF879107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.




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