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Anti-Human TNFa/TNF-alpha Antibody (SAA0415) (HF879107)

Anti-Human TNFa/TNF-alpha Antibody (SAA0415)
Anti-Human TNFa/TNF-alpha Antibody (SAA0415)
Anti-Human TNFa/TNF-alpha Antibody (SAA0415)
Anti-Human TNFa/TNF-alpha Antibody (SAA0415)
Anti-Human TNFa/TNF-alpha Antibody (SAA0415)
Anti-Human TNFa/TNF-alpha Antibody (SAA0415)
Prix(USD) : $
Spécification :
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  • 100ug
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Aperçu
Numéro de catalogueHF879107
Reactivité des espècesHuman
ApplicationsELISA, FCM
Espèce hôteMouse
IsotypeIgG2a, kappa
ID de cloneSAA0415
Clonalité Monoclonal
Cible TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P01375
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 0.09% Sodium azide.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • Anti-Human TNFa/TNF-alpha Antibody (SAA0415)

    Flow-cytometry

    Flow-cytometry using anti-human TNFa antibody.TNFa Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa antibody monoclonal antibody (Catalog # HF879107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human TNFa/TNF-alpha Antibody (SAA0415)

    Flow-cytometry

    Flow-cytometry using anti-human TNFa antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa antibody monoclonal antibody (Catalog # HF879107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human TNFa/TNF-alpha Antibody (SAA0415)

    Flow CytoMetry

    Flow-cytometry using anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog: HF879107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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