Anti-Human CD86/B7-2 Antibody (SAA1397) (HW776207)

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Datasheet

Vista general

Número de catálogo	HW776207
Descripción	Anti-Human CD86/B7-2 Antibody (SAA1397) [SAA1397] (HW776207) is a mouse monoclonal antibody detecting CD86 in ELISA, FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Reactividad de especies	Human
Aplicaciones	ELISA, FCM
Especie huésped	Mouse
Isotipo	IgG2a, kappa
ID de clon	SAA1397
Clonalidad	Monoclonal
Objetivo	BU63, B70, CD28LG2, CTLA-4 counter-receptor B7.2, Activation B7-2 antigen, T-lymphocyte activation antigen CD86, CD86, FUN-1
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P42081
Forma	Liquid
Buffer de almacenamiento	0.01M PBS, pH 7.4, 0.09% Sodium azide. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo	T-lymphocyte activation antigen CD86 (B7-2) is a ~37 kDa protein. Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. 1. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291 2. Freeman, GJ. et al. (1993) Science (New York, N.Y.) 262, 909-11. PMID: 7694363 3. Lanier, LL. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 97-105. PMID: 7527824 4. Schwartz, JC. et al. (2001) Nature 410, 604-8. PMID: 11279501
Nota	For research use only.

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Bioactivity

Detects Human CD86/B7-2 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD86 antibody.Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD86 antibody monoclonal antibody (Catalog # HW776207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD86 antibody.K562 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD86 antibody monoclonal antibody (Catalog # HW776207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.