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Anti-Human CD324/CDH1 Antibody (67A4), APC (HY460137)

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Anti-Human CD324/CDH1 Antibody (67A4), APC

Anti-Human CD324/CDH1 Antibody (67A4), APC

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Aperçu

Numéro de catalogue	HY460137
Description	Anti-Human CD324/CDH1 Antibody (67A4), APC [67A4] (HY460137) is a mouse monoclonal antibody detecting CD324 in FCM, IF. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Reactivité des espèces	Human
Applications	FCM, IF
Espèce hôte	Mouse
Isotype	IgG1, kappa
ID de clone	67A4
Conjugaison	APC
Clonalité	Monoclonal
Cible	CD324, Uvomorulin, Epithelial cadherin, E-cadherin, CDHE, CAM 120/80, UVO, CDH1, Cadherin-1
Niveau d'endotoxines	Please contact with the lab for this information.
Numéro d'accès	P12830
Forme	Liquid
Tampon de stockage	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte	Cadherin-1 (CD324/CDH1) is a ~97 kDa protein. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane. Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques. 1. Meigs, TE. et al. (2002) The Journal of biological chemistry 277, 24594-600. PMID: 11976333 2. Shafraz, O. et al. (2018) eLife 7. PMID: 29999492
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow CytoMetry

Flow-cytometry using APC anti-human CD324 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD324 monoclonal antibody (Catalog: HY460137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Reférences

Fiche technique

Anti-Human CD324/CDH1 Antibody (67A4), APC.pdf

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Anti-Human CD324/CDH1 Antibody (67A4), APC