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Anti-Mouse CD274/PD-L1/B7-H1 Antibody (SAA0359), APC (MV974237)

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Anti-Mouse CD274/PD-L1/B7-H1 Antibody (SAA0359), APC

Anti-Mouse CD274/PD-L1/B7-H1 Antibody (SAA0359), APC

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Aperçu

Numéro de catalogue	MV974237
Description	Anti-Mouse CD274/PD-L1/B7-H1 Antibody (SAA0359), APC [SAA0359] (MV974237) is a mouse monoclonal antibody detecting PD-L1 in ELISA, FCM, IF. Suitable for Mouse. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Reactivité des espèces	Mouse
Applications	ELISA, FCM, IF
Espèce hôte	Mouse
Isotype	VHH, mFc
ID de clone	SAA0359
Conjugaison	APC
Clonalité	Monoclonal
Cible	Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, Cd274, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1
Niveau d'endotoxines	Please contact with the lab for this information.
Numéro d'accès	Q9EP73
Forme	Liquid
Tampon de stockage	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte	Programmed cell death 1 ligand 1 (CD274/PD-L1) is a ~32 kDa protein. Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). CD274 is the therapeutic target of atezolizumab (Tecentriq) and durvalumab (Imfinzi). 1. Tamura, H. et al. (2001) Blood 97, 1809-16. PMID: 11238124 2. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443 3. Wang, S. et al. (2003) The Journal of experimental medicine 197, 1083-91. PMID: 12719480
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using APC anti-mouse CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-mouse CD274 monoclonal antibody (Catalog: MV974237, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Reférences

Fiche technique

Anti-Mouse CD274/PD-L1/B7-H1 Antibody (SAA0359), APC.pdf

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Anti-Mouse CD274/PD-L1/B7-H1 Antibody (SAA0359), APC