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Anti-Human CD40/TNFRSF5 Antibody (4D11), PE (HB782127)

Anti-Human CD40/TNFRSF5 Antibody (4D11), PE
Anti-Human CD40/TNFRSF5 Antibody (4D11), PE
Prix(USD) : $
Spécification :
  • 50T
  • 100T
Nombre :
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Aperçu
Numéro de catalogueHB782127
Description
Anti-Human CD40/TNFRSF5 Antibody (4D11), PE [4D11] (HB782127) is a human monoclonal antibody detecting TNFRSF5 in FCM. Suitable for Human.
Highlights
  • Flow Cytometry — Validated for flow cytometry applications.
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteHuman
IsotypeIgG4, kappa
ID de clone4D11
ConjugaisonPE
Clonalité Monoclonal
Cible Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, TNFRSF5, CDw40, CD40
Niveau d'endotoxines Please contact with the lab for this information.
Numéro d'accès P25942
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte

Tumor necrosis factor receptor superfamily member 5 (CD40/TNFRSF5) is a ~30 kDa protein. Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.

1. Takada, YK. et al. (2019) Journal of immunology (Baltimore, Md. : 1950) 203, 1383-1391. PMID: 31331973
Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human CD40/TNFRSF5 Antibody (4D11), PE

    Flow CytoMetry

    Flow-cytometry using PE anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD40 monoclonal antibody (Catalog: HB782127, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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