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Anti-Human TIGIT Antibody (SAA1407), PE (HS739327)

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Anti-Human TIGIT Antibody (SAA1407), PE

Anti-Human TIGIT Antibody (SAA1407), PE

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100T

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Datasheet

Vista general

Número de catálogo	HS739327
Descripción	Anti-Human TIGIT Antibody (SAA1407), PE [SAA1407] (HS739327) is a mouse monoclonal antibody detecting TIGIT in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Reactividad de especies	Human
Aplicaciones	FCM
Especie huésped	Mouse
Isotipo	IgG2a, kappa
ID de clon	SAA1407
Conjugación	PE
Clonalidad	Monoclonal
Objetivo	VSTM3, TIGIT, V-set and transmembrane domain-containing protein 3, VSIG9, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains
Nivel de endotoxinas	Please contact with the lab for this information.
Número de acceso	Q495A1
Forma	Liquid
Buffer de almacenamiento	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo	T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a ~26 kDa protein. Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. 1. Yu, X. et al. (2009) Nature immunology 10, 48-57. PMID: 19011627 2. Liu, S. et al. (2013) Cell death and differentiation 20, 456-64. PMID: 23154388 3. Li, M. et al. (2014) The Journal of biological chemistry 289, 17647-57. PMID: 24817116 4. Reches, A. et al. (2020) Journal for immunotherapy of cancer 8. PMID: 32503945
Nota	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Imágenes

Flow cytometry

Flow-cytometry PE using anti-human TIGIT antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human TIGIT monoclonal antibody (Catalog HS739327) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Referencias

Datasheet

Anti-Human TIGIT Antibody (SAA1407), PE.pdf

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Anti-Human TIGIT Antibody (SAA1407), PE