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Anti-Human CD20/MS4A1 Antibody (SAA2208), PE (HY257527)

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Anti-Human CD20/MS4A1 Antibody (SAA2208), PE

Anti-Human CD20/MS4A1 Antibody (SAA2208), PE

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100T

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Aperçu

Numéro de catalogue	HY257527
Description	Anti-Human CD20/MS4A1 Antibody (SAA2208), PE [SAA2208] (HY257527) is a human monoclonal antibody detecting MS4A1 in ELISA, FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Flow Cytometry — Validated for flow cytometry applications.
Reactivité des espèces	Human
Applications	ELISA, FCM
Espèce hôte	Human
Isotype	IgG1, kappa
ID de clone	SAA2208
Conjugaison	PE
Clonalité	Monoclonal
Cible	B-lymphocyte surface antigen B1, Membrane-spanning 4-domains subfamily A member 1, MS4A1, Leukocyte surface antigen Leu-16, B-lymphocyte antigen CD20, Bp35, CD20
Niveau d'endotoxines	Please contact with the lab for this information.
Purification	Affinity chromatography.
Numéro d'accès	P11836
Forme	Liquid
Tampon de stockage	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte	B-lymphocyte antigen CD20 (MS4A1) is a ~33 kDa protein. B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. CD20 is the therapeutic target of rituximab (MabThera) and obinutuzumab (Gazyva). 1. Li, H. et al. (2003) The Journal of biological chemistry 278, 42427-34. PMID: 12920111 2. Tedder, TF. et al. (1985) Journal of immunology (Baltimore, Md. : 1950) 135, 973-9. PMID: 3925015 3. Bubien, JK. et al. (1993) The Journal of cell biology 121, 1121-32. PMID: 7684739 4. Polyak, MJ. et al. (2008) The Journal of biological chemistry 283, 18545-52. PMID: 18474602
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using PE anti-Human CD20 antibody. Daudi cells were stained with an irrelevant antibody (Red Histogram) or a PE anti-Human CD20 antibody monoclonal antibody (Catalog # HY257527 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Reférences

Fiche technique

Anti-Human CD20/MS4A1 Antibody (SAA2208), PE.pdf

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Anti-Human CD20/MS4A1 Antibody (SAA2208), PE