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Recombinant Human IL1B/IL1F2 Protein, C-His (HF943012)

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Recombinant Human IL1B/IL1F2 Protein, C-His

Recombinant Human IL1B/IL1F2 Protein, C-His

Recombinant Human IL1B/IL1F2 Protein, C-His

Recombinant Human IL1B/IL1F2 Protein, C-His

Recombinant Human IL1B/IL1F2 Protein, C-His

Recombinant Human IL1B/IL1F2 Protein, C-His

Recombinant Human IL1B/IL1F2 Protein, C-His

Recombinant Human IL1B/IL1F2 Protein, C-His

Recombinant Human IL1B/IL1F2 Protein, C-His

Recombinant Human IL1B/IL1F2 Protein, C-His

Precio(USD): $

Especificación:

50ug
100ug
1mg

Número:

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Vista general
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Referencias
Datasheet

Vista general

Número de catálogo	HF943012
Descripción	Recombinant Human IL1B/IL1F2 Protein, C-His (HF943012) expressed in E. coli, spanning Ala117-Ser269. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P01584
Longitud de proteína	Ala117-Ser269
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	18.33 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Interleukin-1 beta, IL1B, Catabolin, IL1F2, IL-1 beta, IL-1β
Fondo	Interleukin-1 beta (IL1B/IL1F2) is a ~30 kDa protein. Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. IL1B is the therapeutic target of canakinumab (Ilaris). 1. Tominaga, K. et al. (2000) International immunology 12, 151-60. PMID: 10653850 2. Nakahara, H. et al. (2003) Arthritis and rheumatism 48, 1521-9. PMID: 12794819 3. LaRock, CN. et al. (2016) Science immunology 1. PMID: 28331908 4. Van Damme, J. et al. (1985) Nature 314, 266-8. PMID: 3920526 5. Tian, XX. et al. (2021) International endodontic journal 54, 916-925. PMID: 33377178 6. Xia, S. et al. (2021) Nature 593, 607-611. PMID: 33883744
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human IL1B/IL1F2 Protein, C-His
Bioactivity

Detects Rilonacept in indirect ELISAs.
Western blot

Detected by Anti-IL1B/IL1F2 monoclonal antibody (Catalog No: HF943076)
Western blot

Detected by Anti-IL1B/IL1F2 monoclonal antibody (Catalog No: HS856036)
Bioactivity

Detected by Research Grade Anti-Human IL1B/IL1F2 (LY2189102) (Catalog No: HF943076) in indirect ELISA

Referencias

HF943012-SDS-PAGE-1.jpg

Datasheet

Recombinant Human IL1B/IL1F2 Protein, C-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Volume

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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V₂ (Working Vol.)

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Recombinant Human IL1B/IL1F2 Protein, C-His