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Recombinant Human TDP43 Protein, N-His (HC445012)

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Recombinant Human TDP43 Protein, N-His

Recombinant Human TDP43 Protein, N-His

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100ug
1mg

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Aperçu

Numéro de catalogue	HC445012
Description	Recombinant Human TDP43 Protein, N-His (HC445012) expressed in E. coli, spanning Ser104-Pro262. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q13148
Longueur de protéine	Ser104-Pro262
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	20.6 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	TAR DNA-binding protein 43, TARDBP, TDP-43, TDP43
Contexte	TAR DNA-binding protein 43 (TARDBP/TDP43) is a ~44 kDa protein. RNA-binding protein that is involved in various steps of RNA biogenesis and processing. Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs. In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases. Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts. Also regulates mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening. 1. Bhardwaj, A. et al. (2013) Nucleic acids research 41, 5062-74. PMID: 23519609 2. Lukavsky, PJ. et al. (2013) Nature structural & molecular biology 20, 1443-9. PMID: 24240615 3. Kuo, PH. et al. (2014) Nucleic acids research 42, 4712-22. PMID: 24464995 4. Tollervey, JR. et al. (2011) Nature neuroscience 14, 452-8. PMID: 21358640 5. Wang, A. et al. (2018) The EMBO journal 37. PMID: 29438978 6. Izumikawa, K. et al. (2017) Scientific reports 7, 7709. PMID: 28794432 7. Fukushima, M. et al. (2019) FEBS letters 593, 277-287. PMID: 30520513 8. Colombrita, C. et al. (2009) Journal of neurochemistry 111, 1051-61. PMID: 19765185 9. Higashi, S. et al. (2013) Journal of neurochemistry 126, 288-300. PMID: 23398327
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human TDP43 Protein, N-His

Reférences

HC445012-SDS-PAGE-1.jpg

Fiche technique

Recombinant Human TDP43 Protein, N-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

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Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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V₂ (Working Vol.)

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Recombinant Human TDP43 Protein, N-His