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Recombinant Human HIF2A/EPAS1 Protein, N-His (HV758012)

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Recombinant Human HIF2A/EPAS1 Protein, N-His

Recombinant Human HIF2A/EPAS1 Protein, N-His

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100ug
1mg

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Aperçu

Numéro de catalogue	HV758012
Description	Recombinant Human HIF2A/EPAS1 Protein, N-His (HV758012) expressed in E. coli, spanning Leu239-Asn350. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q99814
Longueur de protéine	Leu239-Asn350
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	15.32 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Basic-helix-loop-helix-PAS protein MOP2, bHLHe73, HIF-2-alpha, Member of PAS protein 2, PAS domain-containing protein 2, HIF2A, HLF, EPAS1, Endothelial PAS domain-containing protein 1, EPAS-1, HIF-1-alpha-like factor, Hypoxia-inducible factor 2-alpha, MOP2, HIF2-alpha, BHLHE73, PASD2, Class E basic helix-loop-helix protein 73
Contexte	Endothelial PAS domain-containing protein 1 (HIF2A/EPAS1) is a ~96 kDa protein. Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression.
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human HIF2A/EPAS1 Protein, N-His

Reférences

HV758012-SDS-PAGE-1.jpg

Fiche technique

Recombinant Human HIF2A/EPAS1 Protein, N-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Volume

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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Recombinant Human HIF2A/EPAS1 Protein, N-His