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Recombinant Nipah virus/NiV F/Fusion glycoprotein F0 Protein, N-His (VK521022)

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Recombinant Nipah virus/NiV F/Fusion glycoprotein F0 Protein, N-His

Recombinant Nipah virus/NiV F/Fusion glycoprotein F0 Protein, N-His

Recombinant Nipah virus/NiV F/Fusion glycoprotein F0 Protein, N-His

Recombinant Nipah virus/NiV F/Fusion glycoprotein F0 Protein, N-His

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50ug
100ug
1mg

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Datasheet

Vista general

Número de catálogo	VK521022
Descripción	Recombinant Nipah virus/NiV F/Fusion glycoprotein F0 Protein, N-His (VK521022) expressed in E. coli, spanning Gly131-Thr546. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q9IH63
Longitud de proteína	Gly131-Thr546
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Nipah virus (NiV)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	48.43 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Fusion glycoprotein F0, Protein F, Fusion glycoprotein F2, Fusion glycoprotein F1, F
Fondo	Fusion glycoprotein F0 is a ~60 kDa protein. Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm.
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Nipah virus/NiV F/Fusion glycoprotein F0 Protein, N-His
Western blot

Detected by Anti-Nipah virus fusion protein polyclonal antibody (Catalog No: VK521014)

Referencias

VK521022-SDS-PAGE-1.jpg

Datasheet

Recombinant Nipah virus/NiV F/Fusion glycoprotein F0 Protein, N-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

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Stock Solution

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Working Solution

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Recombinant Nipah virus/NiV F/Fusion glycoprotein F0 Protein, N-His