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Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His (JN868012)

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Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His

Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His

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Número de catálogo	JN868012
Descripción	Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His (JN868012) expressed in E. coli, spanning Ala1160-Asp1521. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P11311
Longitud de proteína	Ala1160-Asp1521
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	40.03 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Adhesin P1, Attachment protein, Cytadhesin P1, mgpA, MPN_141, MP013
Fondo	Adhesin P1 (mgpA) is a ~176 kDa protein. Part of major adhesin Nap complex of the attachment organelle which mediates attachment to host ciliated epithelium and plays a major role in gliding motility. This protein and P40/P90 mutually stabilize each other. The 3 adhesin proteins are required for formation of the attachment organelle. 1. Vizarraga, D. et al. (2020) Nature communications 11, 5188. PMID: 33057023 2. Vizarraga, D. et al. (2025) PLoS pathogens 21, e1012973. PMID: 40153444 3. Waldo, RH. et al. (2006) Journal of bacteriology 188, 569-75. PMID: 16385047
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His

Referencias

JN868012-SDS-PAGE-1.jpg

Datasheet

Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His.pdf

Formula

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Stock Solution

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Working Solution

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Recombinant Mycoplasma pneumoniae mgpA/Adhesin P1 Protein, N-His