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Recombinant Mouse TP53/p53 Protein, N-His (MY039012)

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Recombinant Mouse TP53/p53 Protein, N-His

Recombinant Mouse TP53/p53 Protein, N-His

Recombinant Mouse TP53/p53 Protein, N-His

Recombinant Mouse TP53/p53 Protein, N-His

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50ug
100ug
1mg

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Datasheet

Vista general

Número de catálogo	MY039012
Descripción	Recombinant Mouse TP53/p53 Protein, N-His (MY039012) expressed in E. coli, spanning Met1-Asp390. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P02340
Longitud de proteína	Met1-Asp390
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Mus musculus (Mouse)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	45.62 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Antigen NY-CO-13, Cellular tumor antigen p53, P53, Tumor suppressor p53, TP53, Phosphoprotein p53
Fondo	Cellular tumor antigen p53 (TP53) is a ~43 kDa protein. Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence. Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. 1. Allton, K. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 11612-6. PMID: 19556538 2. Huarte, M. et al. (2010) Cell 142, 409-19. PMID: 20673990 3. Vaseva, AV. et al. (2012) Cell 149, 1536-48. PMID: 22726440 4. Miki, T. et al. (2013) Nature communications 4, 2444. PMID: 24051492
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Mouse TP53/p53 Protein, N-His
Bioactivity

Detected by Anti-Mouse TP53/p53 Antibody (PAb242) (Cat No.: MY039013) in indirect ELISA.

Referencias

MY039012-SDS-PAGE-1.jpg

Datasheet

Recombinant Mouse TP53/p53 Protein, N-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

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Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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Recombinant Mouse TP53/p53 Protein, N-His