Recombinant Toxoplasma gondii SAG1/Major surface antigen p30 Protein, N-His-SUMO (JN902012)

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Vista general

Número de catálogo	JN902012
Descripción	Recombinant Toxoplasma gondii SAG1/Major surface antigen p30 Protein, N-His-SUMO (JN902012) expressed in E. coli, spanning Ser48-Leu198. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P13664
Longitud de proteína	Ser48-Leu198
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Toxoplasma gondii
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	28.32 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Major surface antigen p30
Fondo	Major surface antigen p30 (SAG1) is a ~34 kDa protein. Mediates binding to host cells and is especially important for parasite attachment and fitness in IFN-gamma/IFNG-stimulated host cells. May regulate binding between human S100A6 and vimentin (VIM) for cytoskeleton organization during the early stages of invasion into host cells. Promotes TNF secretion in host cells probably by enhancing binding between human VIM and PRKCQ and activating NF-kappa-B pathway. Promotes viability of host cells to satisfy the survival needs of parasites through the interaction with human RACK1. 1. Robinson, SA. et al. (2004) Parasitology 128, 391-6. PMID: 15151144 2. Cohen, O. et al. (2025) Infection and immunity 93, e0001025. PMID: 40607979 3. Zhou, LJ. et al. (2021) iScience 24, 103514. PMID: 34950858 4. Wang, D. et al. (2024) Acta tropica 251, 107112. PMID: 38157925
Nota	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Toxoplasma gondii SAG1/Major surface antigen p30 Protein, N-His-SUMO

Referencias

Datasheet

Recombinant Toxoplasma gondii SAG1/Major surface antigen p30 Protein, N-His-SUMO.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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