OMIP Reviews | OMIP-020: 11-Colour Panel for In-Depth Analysis of γδ T Cell Phenotype and Immune Responses

Date de publication : 2026-04-16 Nombre de vues : 7

Exploring the Immune Function of γδ T Cells

γδ T cells play a crucial role in immune responses, particularly in cancer immune surveillance, infection immunity, and immunosenescence. Despite their importance, precise analysis of these subsets has remained a significant technical challenge.

Innovative Features and Advantages of OMIP-020

OMIP-020 offers an innovative 11-colour flow cytometry panel that allows for comprehensive profiling of γδ T cell subsets. By distinguishing between Vδ1 and Vδ2 subsets and incorporating functional markers such as CD16, CD27, and CD28, this panel enables a detailed analysis of γδ T cell activation, memory characteristics, and functional differentiation.

Opening New Avenues in Immune Research and Expanding Application Potential

OMIP-020 not only provides tools for detailed subset analysis and functional assessment but also allows for deeper exploration of the diverse roles of γδ T cells in immune responses. Whether used in cancer immune surveillance, infection research, or the study of immunosenescence, OMIP-020 offers strong technical support to researchers.

OMIP-020 offers advanced technology for the efficient analysis of γδ T cells, revealing their critical roles in immune surveillance, infection immunity, cancer immunology, and immunosenescence. With its 11-colour labeling capabilities, the panel provides comprehensive support for profiling γδ T cell subsets and their functional properties, driving forward research in these important areas.

1. OMIP-020Panel

Target	Fluorochrome	Function	abinScience Recommendation
Live/Dead	EMA	Exclude dead cells	—
CD3	Alexa 700	T cell lineage marker	View CD3 antibodies
CD4	PE-Cy7		View CD4 antibodies
CD8	APC-H7		View CD8 antibodies
TCR γ/δ	Purified-Pacific Orange	γδ T cell marker	View TCR antibodies
TCR-Vδ1	FITC
TCR-Vδ2	PerCP
CD16	BV 711	Differentiation/activation/memory phenotype markers	View CD16 antibodies
CD27	APC		View CD27 antibodies
CD28	PE		View CD28 antibodies
CD45RA	Pacific Blue		—

2. Gate Logic

Overview of OMIP-020 Gating

Figure 1. Overview of OMIP-020 Gating Strategy

Main Cell Population Identification

Exclude aggregates, exclude dead cells, and gate on live CD3+ T cells.

T Cell Subset Analysis

Use CD4/CD8 to identify CD4+ T cells, CD8+ T cells, and CD4-CD8- T cells.

γδ T Cell Profiling

Use TCR-Vδ1 and TCR-Vδ2 to identify and distinguish between Vδ1+ and Vδ2+ γδ T cells.

Detailed γδ T Cell Phenotype Analysis

Analyze activation status, memory characteristics, and functional differentiation of Vδ1+ and Vδ2+ γδ T cells using markers such as CD16, CD27, CD28, and CD45RA.

3. Experimental Results

1). Gate the main cell population from PBMCs by excluding aggregates and dead cells, then gate on live CD3+ T cells. Use CD4/CD8 markers to further distinguish between CD4+ T, CD8+ T, and CD4-CD8- T cells.

2). Within the CD4-CD8- T cell population, use TCR γ/δ to identify γδ T cells, followed by distinguishing Vδ1+ and Vδ2+ T cells using TCR-Vδ1 and TCR-Vδ2 markers.

3). Analyze the activation status, memory features, and functional differentiation of Vδ1+ and Vδ2+ T cells using CD16, CD27, CD28, and CD45RA markers.

4. Panel Interpretation

4.1 Filling the Gap in γδ T Cell Subset Research

OMIP-020 is the first multicolour flow cytometry panel specifically designed for γδ T cells, filling an important gap in the study of these immune subsets. By providing detailed characterization of γδ T cell subsets (such as Vδ1 and Vδ2), it has become an essential tool for understanding the key roles γδ T cells play in immune responses. In particular, it offers new insights into immune surveillance, cancer immune evasion, infection immunity, and immunosenescence, providing researchers with new perspectives and technical support.

4.2 Comprehensive Subset and Functional Phenotype Analysis

OMIP-020 addresses both "subset differentiation" and "functional state assessment," meeting the core needs of γδ T cell research. The panel not only allows precise distinction between major γδ T cell subsets (Vδ1 and Vδ2) but also evaluates their activation status, memory features, and functional differentiation through markers such as CD16, CD27, CD28, and CD45RA. This comprehensive analysis provides powerful tools for understanding the multiple functions of γδ T cells, particularly their roles in infection, cancer immune surveillance, and immunosenescence.

5. Applications

Basic Immunology Research Immune Therapy Monitoring Mechanisms of Immune Cell Differentiation Cancer Immunology Immune Monitoring in Disease States Aging and Immunology Research

6. Conclusion

OMIP-020 provides a new tool for γδ T cell research, offering comprehensive coverage of subset differentiation and functional phenotype analysis. By precisely distinguishing Vδ1 and Vδ2 subsets and combining functional markers such as CD16, CD27, and CD28, researchers can deeply analyze γδ T cell activation, memory features, and functional differentiation. This panel has broad applications in cancer immune surveillance, infection immunity, and immunosenescence research.

Get OMIP-020 Compatible Flow Cytometry Antibodies

abinScience provides validated Flow Cytometry Antibodies covering key targets in this panel, supporting your γδ T cell research

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References

[1] Wistuba-Hamprecht K, Pawelec G, Derhovanessian E. OMIP-020: Phenotypic characterization of human γδ T-cells by multicolor flow cytometry. Cytometry A. 2014 Jun;85(6):522-4.

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