FAQ

Choose the right blocking reagent for your assay. Compare BSA, milk, normal serum, casein, and protein-free blockers for WB, IHC, IF, and ELISA with troubleshooting tips.

Select the right WB loading control by molecular weight: beta-Actin, GAPDH, Vinculin, Lamin B1, Histone H3. Avoid overlapping bands with this MW-based selection guide.

Learn how to select non-competing capture and detection antibody pairs for sandwich ELISA. Covers epitope matching rules, coating optimization, checkerboard titration, and troubleshooting.

Compare RIPA, NP-40, urea, and specialized lysis buffers for WB, IP, ELISA, and phosphoproteomics. Includes recipes, tissue-specific tips, and troubleshooting guide.

Complete ChIP protocol with step-by-step instructions for X-ChIP and N-ChIP. Includes sonication optimization, antibody selection criteria, and troubleshooting common problems.

Troubleshoot common Co-IP problems: weak signal, high background bands, and failed reverse IPs. Includes optimized protocol, Protein A vs G selection guide, and antibody tips.

A practical guide to isotype controls in flow cytometry. Covers what isotype controls measure, how they differ from FMO controls, when they are appropriate, common misuses, and recommendations for proper gating strategies in multicolor flow cytometry panels.

Learn what research biosimilars are, how they compare to originator drugs, and their applications in PK/ADA assays, functional studies, and preclinical models. Browse 2,700+ research biosimilar antibodies at abinScience covering PD-1, HER2, CD20, TNF-alpha and more.

Protein quantification is vital in biochemistry, medicine, and food science for diagnostics and quality control. Methods like Lowry, BCA, UV spectrophotometry, and Kjeldahl offer unique approaches to measure protein concentration, each with specific principles, applications, and precautions. These techniques ensure accurate and reliable results for diverse samples, from serum to food, supporting research and industry needs.

UniProt is a comprehensive protein database collaboratively maintained by EMBL-EBI, SIB, and NCBI. It comprises UniProtKB, Proteomes, UniRef, and UniParc, offering extensive protein sequences, functional annotations, and structural data. With integrated tools like BLAST and Align, UniProt supports advanced research in bioinformatics, molecular biology, and proteomics.