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| カタログ番号 | JN084014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Escherichia coli O127:H6 (strain E2348/69 / EPEC) | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| アイソタイプ | IgG | ||||||||
| クローン性 | Polyclonal | ||||||||
| 免疫原 | E. coli - derived recombinant Escherichia coli eae/Intimin (Lys450-Gln655). | ||||||||
| ターゲット | Intimin, Attaching and effacing protein, Eae protein, eae, eaeA, E2348C_3939 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P19809 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Intimin (eae) is a ~102 kDa protein. An inverse autotransporter. Adhesin, which mediates attachment to the human intestine epithelial cells. Necessary for the production of attaching and effacing lesions on infected human tissue culture cells. Anchored to the outer membrane by binding to peptidoglycan (PGN) via its periplasmic domain, thus helping in receptor interactions during host invasion (Probable). PGN-binding may also aid in resisting mechanical and chemical stress during transit of the bacterium through the gastrointestinal tract of the host (Probable). 1. Leo, JC. et al. (2015) Molecular microbiology 95, 80-100. PMID: 25353290 2. Weikum, J. et al. (2020) Scientific reports 10, 21249. PMID: 33277518 3. Jerse, AE. et al. (1990) Proceedings of the National Academy of Sciences of the United States of America 87, 7839-43. PMID: 2172966 4. Batchelor, M. et al. (2000) The EMBO journal 19, 2452-64. PMID: 10835344 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-Escherichia coli eae/Intimin polyclonal antibody at 1ug/mL on recombinant Escherichia coli eae/Intimin (Catalog No. JN084012).
Western blot analysis was performed using anti-EAE polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Escherichia coli eae/Intimin (Catalog No: JN084012)
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中国武漢市深敦寺路666号C棟、武漢、430206
