Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody (JN045014)

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概要

カタログ番号

JN045014

説明

Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody (JN045014) is a rabbit polyclonal antibody detecting cas9 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

種反応性

Streptococcus pyogenes serotype M1

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

アイソタイプ

IgG

クローン性

Polyclonal

免疫原

E. coli - derived recombinant Streptococcus pyogenes Cas9 (Met1-Asp1368).

ターゲット

CRISPR-associated endonuclease Cas9/Csn1, 3.1.-.-, SpCas9, SpyCas9, cas9, csn1, SPy_1046

精製

Purified by antigen affinity column.

アクセッション番号

Q99ZW2

RRID

Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody (abinScience Cat# JN045014, RRID:AB_3724889)

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

CRISPR-associated endonuclease Cas9/Csn1 is a ~158 kDa protein. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing.

1. Deltcheva, E. et al. (2011) Nature 471, 602-7. PMID: 21455174

2. Fonfara, I. et al. (2014) Nucleic acids research 42, 2577-90. PMID: 24270795

3. Sternberg, SH. et al. (2014) Nature 507, 62-7. PMID: 24476820

注意事項

For research use only.

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