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| カタログ番号 | YP539203 | ||||
|---|---|---|---|---|---|
| 説明 |
Anti-dsDNA Antibody (3E10#) [3E10#] (YP539203) is a mouse monoclonal antibody detecting dsDNA in DB, ELISA, IF.
Highlights
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| アプリケーション | DB, ELISA, IF | ||||
| 宿主種 | Mouse | ||||
| アイソタイプ | IgG2a, kappa | ||||
| クローンID | 3E10# | ||||
| クローン性 | Monoclonal | ||||
| ターゲット | DNA, dsDNA | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| RRID | Anti-dsDNA Antibody (3E10#) (abinScience Cat# YP539203, RRID:AB_3725561) |
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| 形態 | Liquid | ||||
| 保存バッファー | 0.94 mM Citric acid monohydrate, 16.7mM Trisodium citrate dihydrate, 6.2% sucrose, pH 6.5, 0.01% Tween 80 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 注意事項 | For research use only. |
SDS-PAGE for Anti-dsDNA Antibody (3E10#).
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting dsDNA (YP539203) overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Mouse IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of dsDNA was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
MG63 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting dsDNA (YP539203) overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Mouse IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of dsDNA was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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