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| カタログ番号 | HV974063 | ||||||
|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human CD274/PD-L1/B7-H1 Antibody (SAA2018) [SAA2018] (HV974063) is a human monoclonal antibody detecting B7-H1 in ELISA, WB. Suitable for Human.
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| 種反応性 | Human | ||||||
| アプリケーション | ELISA, WB | ||||||
| 宿主種 | Human | ||||||
| アイソタイプ | VHH-hFc | ||||||
| クローンID | SAA2018 | ||||||
| クローン性 | Monoclonal | ||||||
| ターゲット | B7-H1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, PDCD1L1, B7 homolog 1, PDCD1LG1, PDL1, hPD-L1, Programmed death ligand 1, B7H1, PD-L1, CD274 | ||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||
| 純度 | >95% as determined by SDS-PAGE. | ||||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||||
| アクセッション番号 | Q9NZQ7 | ||||||
| RRID | Anti-Human CD274/PD-L1/B7-H1 Antibody (SAA2018) (abinScience Cat# HV974063, RRID:AB_3727930) |
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| 形態 | Liquid | ||||||
| 保存バッファー | 0.01M PBS, pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| 背景 | Programmed cell death 1 ligand 1 (CD274/PD-L1) is a ~33 kDa protein. Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis. CD274 is the therapeutic target of atezolizumab (Tecentriq) and durvalumab (Imfinzi). 1. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443 2. Mezzadra, R. et al. (2017) Nature 549, 106-110. PMID: 28813410 3. Burr, ML. et al. (2017) Nature 549, 101-105. PMID: 28813417 4. Huang, X. et al. (2019) Cancer immunology research 7, 1580-1590. PMID: 31399419 5. Kennedy, A. et al. (2023) The EMBO journal 42, e111556. PMID: 36727298 6. Dong, H. et al. (1999) Nature medicine 5, 1365-9. PMID: 10581077 7. Hou, J. et al. (2020) Nature cell biology 22, 1264-1275. PMID: 32929201 | ||||||
| 注意事項 | For research use only. |
Detects Human CD274/PD-L1/B7-H1 in indirect ELISAs.
Flow-cytometry using APC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD274 monoclonal antibody (Catalog: HV974063, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using FITC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD274 monoclonal antibody (Catalog: HV974063, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD274 monoclonal antibody (Catalog: HV974063, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CD274 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD274 monoclonal antibody (Catalog: HV974063) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot analysis was performed using anti-CD274/PD-L1/B7-H1 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant mouse CD274/PD-L1/B7-H1 (Catalog No: MV974011), 50ng
Lane 2: recombinant mouse CD274/PD-L1/B7-H1 (Catalog No: MV974011), 30ng
Lane 3: recombinant mouse CD274/PD-L1/B7-H1 (Catalog No: MV974011), 20ng
Lane 4: recombinant mouse CD274/PD-L1/B7-H1 (Catalog No: MV974011), 10ng
SDS-PAGE for Anti-Human CD274/PD-L1/B7-H1 Antibody (SAA2018)
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