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Anti-CPV-2 Capsid protein VP2 Polyclonal Antibody (VK761014)

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概要
カタログ番号VK761014
説明
Anti-CPV-2 Capsid protein VP2 Polyclonal Antibody (VK761014) is a rabbit polyclonal antibody detecting CPV-2 Capsid protein VP2 in WB, IHC, ELISA.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
種反応性Canine parvovirustype 2 (CPV-2)
アプリケーションWB, IHC, ELISA
宿主種Rabbit
アイソタイプIgG
Canine parvovirustype 2 (isolate Dog/United States/CPV-d/1988) (CPV-2)
クローン性 Polyclonal
免疫原 E. coli - derived recombinant CPV-2 Capsid protein VP2 (Phe188-Tyr727(Asn518Glu)).
ターゲット VP1
エンドトキシンレベル Please contact with the lab for this information.
純度 >90% as determined by SDS-PAGE.
精製 Purified by antigen affinity column.
アクセッション番号 P17455
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名Capsid protein VP1, Coat protein VP1
背景

Capsid protein VP1 is a ~80 kDa protein. Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptor TFRC. This attachment induces virion internalization predominantly through clathrin-endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s).

注意事項 For research use only.
イメージ
参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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