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Каталожный номерMB840014
Описание
Anti-Mouse NT-proBNP Polyclonal Antibody (MB840014) is a rabbit polyclonal antibody detecting NT-proBNP in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовMouse
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Mouse NT-proBNP (Tyr27-Leu121).
Цель 5 kDa cardiac natriuretic peptide, BNP, BNP(77-121), Brain natriuretic factor prohormone, Brain natriuretic peptide, Brain natriuretic peptide 45, Gamma-brain natriuretic peptide, Iso-ANP, Natriuretic peptides B, Nppb, preproBNP, proBNP
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >95% as determined by SDS-PAGE.
Очистка Purified by antigen affinity column.
Номер доступа P40753
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Информация об использовании продукта
Применение Разбавление
ELISA 1:2000-1:20000
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Natriuretic peptides B (NPPB/BNP) is a ~14 kDa protein. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3.

1. Koller, KJ. et al. (1991) Science (New York, N.Y.) 252, 120-3. PMID: 1672777
2. Heublein, DM. et al. (2007) Hypertension (Dallas, Tex. : 1979) 49, 1114-9. PMID: 17372040
3. Yoshimura, M. et al. (1991) Circulation 84, 1581-8. PMID: 1914098
4. Jensen, KT. et al. (1998) The American journal of physiology 274, F63-72. PMID: 9458824
5. Liang, F. et al. (2007) Journal of the American College of Cardiology 49, 1071-8. PMID: 17349887
6. Ralat, LA. et al. (2011) The Journal of biological chemistry 286, 4670-9. PMID: 21098034
7. Huntley, BK. et al. (2015) Circulation. Heart failure 8, 89-97. PMID: 25339504
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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