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Каталожный номерVK544034
Описание
Anti-NDV Nucleoprotein Polyclonal Antibody (VK544034) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB. Suitable for Newcastle disease virus (NDV) (Avian orthoavulavirus 1).
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовNewcastle disease virus (NDV) (Avian orthoavulavirus 1)
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant NDV Nucleoprotein (Pro45-Arg398).
Цель N, NP, Nucleocapsid protein, Nucleoprotein, Protein N
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >95% as determined by SDS-PAGE.
Очистка Purified by antigen affinity column.
Номер доступа Q99FY3
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Информация об использовании продукта
Применение Разбавление
ELISA 1:2000-1:20000
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Nucleoprotein (NP) is a ~56 kDa protein. Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins.

Примечание For research use only.
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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