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Anti-Mouse MCM2 Polyclonal Antibody (MW401014)

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Vista general
Número de catálogoMW401014
Descripción
Anti-Mouse MCM2 Polyclonal Antibody (MW401014) is a rabbit polyclonal antibody detecting MCM2 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Mouse MCM2 (Ser181-Lys447).
Objetivo BM28, CCNL1, CDCL1, DNA replication licensing factor MCM2, EC:3.6.4.12, KIAA0030, MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P97310
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

DNA replication licensing factor MCM2 is a ~101 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

1. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425
2. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004
3. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328
4. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232
5. Todorov, IT. et al. (1994) Journal of cell science 107 ( Pt 1), 253-65. PMID: 8175912
6. Gao, J. et al. (2015) PloS one 10, e0133522. PMID: 26196677
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse MCM2 Polyclonal Antibody

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