Anti-Mouse MCM2 Polyclonal Antibody (MW401014)

Name: Anti-Mouse MCM2 Polyclonal Antibody
Brand: abinScience
SKU: MW401014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Fiche technique
SDS

Aperçu

Numéro de catalogue

MW401014

Description

Anti-Mouse MCM2 Polyclonal Antibody (MW401014) is a rabbit polyclonal antibody detecting MCM2 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Mouse MCM2 (Ser181-Lys447).

Cible

BM28, CCNL1, CDCL1, DNA replication licensing factor MCM2, EC:3.6.4.12, KIAA0030, MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P97310

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Contexte

DNA replication licensing factor MCM2 is a ~101 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

1. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

2. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

3. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

4. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

5. Todorov, IT. et al. (1994) Journal of cell science 107 ( Pt 1), 253-65. PMID: 8175912

6. Gao, J. et al. (2015) PloS one 10, e0133522. PMID: 26196677

Note

For research use only

Images

Reférences

Fiche technique

Anti-Mouse MCM2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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