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Обзор
Каталожный номерMB244014
Описание
Anti-Mouse RAB5A Polyclonal Antibody (MB244014) is a rabbit polyclonal antibody detecting RAB5A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Mouse RAB5A (Asn16-Leu181).
Цель EC:3.6.5.2, RAB5, RAB5A, Ras-related protein Rab-5A
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа Q9CQD1
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Фон

Ras-related protein Rab-5A (RAB5A) is a ~23 kDa protein. The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes and involved in early endocytic trafficking. Required for EEA1 recruitment to early endosomes. Recruits FERRY complex to early endosomes, where FERRY links early endosomes with a subgroup of mRNAs to enable mRNA intracellular distribution.

1. Hoffenberg, S. et al. (2000) The Journal of biological chemistry 275, 24661-9. PMID: 10818110
2. Fouraux, MA. et al. (2004) Molecular biology of the cell 15, 611-24. PMID: 14617813
3. Zhu, G. et al. (2004) Nature structural & molecular biology 11, 975-83. PMID: 15378032
4. Haas, AK. et al. (2005) Nature cell biology 7, 887-93. PMID: 16086013
5. Hunker, CM. et al. (2006) Biochemical and biophysical research communications 340, 967-75. PMID: 16410077
6. Fuchs, E. et al. (2007) The Journal of cell biology 177, 1133-43. PMID: 17562788
7. Quentin, D. et al. (2023) Molecular cell 83, 1856-1871.e9. PMID: 37267906
8. Gauthier-Campbell, C. et al. (2004) Molecular biology of the cell 15, 2205-17. PMID: 14978216
9. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413
Примечание For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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