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Anti-Mouse PGK1 Polyclonal Antibody (MF674014)

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Número de catálogoMF674014
Descripción
Anti-Mouse PGK1 Polyclonal Antibody (MF674014) is a rabbit polyclonal antibody detecting PGK1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Mouse PGK1 (Met1-Val417).
Objetivo Cell migration-inducing gene 10 protein, EC:2.7.11.1, EC:2.7.2.3, PGK1, PGKA, PRP 2, Phosphoglycerate kinase 1, Primer recognition protein 2
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P09411
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

Phosphoglycerate kinase 1 (PGK1) is a ~44 kDa protein. Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl-coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis. May play a role in sperm motility.

1. Bollong, MJ. et al. (2018) Nature 562, 600-604. PMID: 30323285
2. Fujii, H. et al. (1980) The Journal of biological chemistry 255, 6421-3. PMID: 7391028
3. Gondeau, C. et al. (2008) Nucleic acids research 36, 3620-9. PMID: 18463139
4. Jindal, HK. et al. (1990) The Journal of biological chemistry 265, 6540-3. PMID: 2324090
5. Li, X. et al. (2016) Molecular cell 61, 705-719. PMID: 26942675
6. Chen, Z. et al. (2023) Nature communications 14, 1121. PMID: 36849569
7. Liu, XX. et al. (2016) Human reproduction (Oxford, England) 31, 273-9. PMID: 26677959
Nota For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse PGK1 Polyclonal Antibody

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