Anti-Mouse PGK1 Polyclonal Antibody (MF674014)

Name: Anti-Mouse PGK1 Polyclonal Antibody
Brand: abinScience
SKU: MF674014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

MF674014

Description

Anti-Mouse PGK1 Polyclonal Antibody (MF674014) is a rabbit polyclonal antibody detecting PGK1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Mouse PGK1 (Met1-Val417).

Cible

Cell migration-inducing gene 10 protein, EC:2.7.11.1, EC:2.7.2.3, PGK1, PGKA, PRP 2, Phosphoglycerate kinase 1, Primer recognition protein 2

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P09411

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Contexte

Phosphoglycerate kinase 1 (PGK1) is a ~44 kDa protein. Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl-coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis. May play a role in sperm motility.

1. Bollong, MJ. et al. (2018) Nature 562, 600-604. PMID: 30323285

2. Fujii, H. et al. (1980) The Journal of biological chemistry 255, 6421-3. PMID: 7391028

3. Gondeau, C. et al. (2008) Nucleic acids research 36, 3620-9. PMID: 18463139

4. Jindal, HK. et al. (1990) The Journal of biological chemistry 265, 6540-3. PMID: 2324090

5. Li, X. et al. (2016) Molecular cell 61, 705-719. PMID: 26942675

6. Chen, Z. et al. (2023) Nature communications 14, 1121. PMID: 36849569

7. Liu, XX. et al. (2016) Human reproduction (Oxford, England) 31, 273-9. PMID: 26677959

Note

For research use only

Images

Reférences

Fiche technique

Anti-Mouse PGK1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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