Anti-Mouse CYP24A1 Polyclonal Antibody (MC495014)

Name: Anti-Mouse CYP24A1 Polyclonal Antibody
Brand: abinScience
SKU: MC495014
Price: 168 USD
Availability: InStock

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Especificación:

50ug
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Referencias
Datasheet
SDS

Vista general

Número de catálogo

MC495014

Descripción

Anti-Mouse CYP24A1 Polyclonal Antibody (MC495014) is a rabbit polyclonal antibody detecting CYP24A1 in ELISA, IHC, WB. Suitable for Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Mouse CYP24A1 (Ser69-Arg514).

Objetivo

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial, 24-OHase, CYP24, CYP24A1, Cytochrome P450 24A1, Cytochrome P450-CC24, EC:1.14.15.16, Vitamin D(3) 24-hydroxylase

Nivel de endotoxinas

Please contact with the lab for this information.

Purificación

Purified by antigen affinity column.

Número de acceso

Q64441

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Fondo

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial (CYP24A1) is a ~58 kDa protein. A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid. With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product. Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol.

1. Sakaki, T. et al. (2000) European journal of biochemistry 267, 6158-65. PMID: 11012668

2. Sakaki, T. et al. (2005) Frontiers in bioscience : a journal and virtual library 10, 119-34. PMID: 15574355

3. Hamamoto, H. et al. (2006) Molecular pharmacology 70, 120-8. PMID: 16617161

4. Tieu, EW. et al. (2014) The FEBS journal 281, 3280-96. PMID: 24893882

5. Roizen, JD. et al. (2018) The Journal of clinical investigation 128, 1913-1918. PMID: 29461981

6. Beckman, MJ. et al. (1996) Biochemistry 35, 8465-72. PMID: 8679605

Nota

For research use only

Imágenes

Referencias

Datasheet

Anti-Mouse CYP24A1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo