Anti-CD3 epsilon Recombinant Antibody (SRR2880) (HY057073)

Name: Anti-CD3 epsilon Recombinant Antibody (SRR2880)
Brand: abinScience
SKU: HY057073
Price: 191 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD): --

Especificación:

50ul
100ul

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo	HY057073
Descripción	Anti-CD3 epsilon Recombinant Antibody (SRR2880) (HY057073) is a rabbit monoclonal antibody detecting CD3 epsilon in IF, FCM, WB. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications.
Reactividad de especies	Human
Aplicaciones	IF, FCM, WB
Especie huésped	Rabbit
Clonalidad	Monoclonal
Isotipo	IgG
ID de clon	SRR2880
Aplicaciones probadas	IF, FCM, WB
Objetivo	CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E
Nivel de endotoxinas	< 10 EU/mg as determined by LAL method.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P07766
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo	T-cell surface glycoprotein CD3 epsilon chain (T3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,T-cell surface glycoprotein CD3 epsilon chain) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. 1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938 2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002 3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098 4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325 5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660
Nota	For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-CD3 epsilon monoclonal antibody at 1 ug/mL on various samples.
Lane 1: Jurkat cell lysate
Immunofluorescence

Jurkat cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CD3 epsilon (HY057073) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of CD3 epsilon was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow cytometry

Flow-cytometry using anti-Human CD3 epsilon antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human CD3 epsilon monoclonal antibody (Catalog HY057073, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

Referencias

Datasheet

Anti-CD3 epsilon Recombinant Antibody (SRR2880).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-CD3 epsilon Recombinant Antibody (SRR2880) (HY057073)

Vista general

Imágenes

Referencias

Datasheet

SDS