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| カタログ番号 | HC273013 |
|---|---|
| 説明 |
Anti-Human CD69 Recombinant Antibody (SRR0102) (HC273013) is a rabbit monoclonal antibody detecting CD69 in IF, FCM. Suitable for Human.
Highlights
|
| 種反応性 | Human |
| アプリケーション | IF, FCM |
| 宿主種 | Rabbit |
| クローン性 | Monoclonal |
| アイソタイプ | IgG |
| クローンID | SRR0102 |
| テスト済みアプリケーション | IF, FCM |
| ターゲット | AIM, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, CD69, CLEC2C, EA1, Early T-cell activation antigen p60, Early activation antigen CD69, GP32/28, Leukocyte surface antigen Leu-23, MLR-3 |
| エンドトキシンレベル | < 10 EU/mg as determined by LAL method. |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | Q07108 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 背景 | Early activation antigen CD69 is a ~22 kDa protein. Transmembrane protein expressed mainly on T-cells resident in mucosa that plays an essential role in immune cell homeostasis. Rapidly expressed on the surface of platelets, T-lymphocytes and NK cells upon activation by various stimuli, such as antigen recognition or cytokine signaling, stimulates different signaling pathways in different cell types. Negatively regulates Th17 cell differentiation through its carbohydrate dependent interaction with galectin-1/LGALS1 present on immature dendritic cells. Association of CD69 cytoplasmic tail with the JAK3/STAT5 signaling pathway regulates the transcription of RORgamma/RORC and, consequently, differentiation toward the Th17 lineage. Also acts via the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells. 1. de la Fuente, H. et al. (2014) Molecular and cellular biology 34, 2479-87. PMID: 24752896 2. Lin, CR. et al. (2015) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29, 5006-17. PMID: 26296369 3. Jiménez-Fernández, M. et al. (2022) Cellular and molecular life sciences : CMLS 79, 468. PMID: 35930205 4. Jiang, Y. et al. (2021) Clinical and translational medicine 11, e563. PMID: 34841721 5. Chen, H. et al. (2023) eLife 12. PMID: 37039481 |
| 注意事項 | For research use only. |
Raji cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CD69 (HC273013) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of CD69 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow-cytometry using anti-Human CD69 antibody. Permeabilized Transfected cells and Control cells were stained with an anti-Human CD69 monoclonal antibody (Catalog HC273013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.
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