Anti-Human CD69 Recombinant Antibody (SRR0102) (HC273013)

Name: Anti-Human CD69 Recombinant Antibody (SRR0102)
Brand: abinScience
SKU: HC273013
Price: 191 USD
Availability: InStock

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50ul
100ul

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Каталожный номер	HC273013
Описание	Anti-Human CD69 Recombinant Antibody (SRR0102) (HC273013) is a rabbit monoclonal antibody detecting CD69 in IF, FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications.
Реактивность видов	Human
Применения	IF, FCM
Хозяинский вид	Rabbit
Клоональность	Monoclonal
Изотип	IgG
ID клона	SRR0102
Проверенные приложения	IF, FCM
Цель	AIM, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, CD69, CLEC2C, EA1, Early T-cell activation antigen p60, Early activation antigen CD69, GP32/28, Leukocyte surface antigen Leu-23, MLR-3
Уровень эндотоксинов	< 10 EU/mg as determined by LAL method.
Чистота	>95% as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	Q07108
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон	Early activation antigen CD69 is a ~22 kDa protein. Transmembrane protein expressed mainly on T-cells resident in mucosa that plays an essential role in immune cell homeostasis. Rapidly expressed on the surface of platelets, T-lymphocytes and NK cells upon activation by various stimuli, such as antigen recognition or cytokine signaling, stimulates different signaling pathways in different cell types. Negatively regulates Th17 cell differentiation through its carbohydrate dependent interaction with galectin-1/LGALS1 present on immature dendritic cells. Association of CD69 cytoplasmic tail with the JAK3/STAT5 signaling pathway regulates the transcription of RORgamma/RORC and, consequently, differentiation toward the Th17 lineage. Also acts via the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells. 1. de la Fuente, H. et al. (2014) Molecular and cellular biology 34, 2479-87. PMID: 24752896 2. Lin, CR. et al. (2015) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29, 5006-17. PMID: 26296369 3. Jiménez-Fernández, M. et al. (2022) Cellular and molecular life sciences : CMLS 79, 468. PMID: 35930205 4. Jiang, Y. et al. (2021) Clinical and translational medicine 11, e563. PMID: 34841721 5. Chen, H. et al. (2023) eLife 12. PMID: 37039481
Примечание	For research use only.

Изображения

Immunofluorescence

Raji cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CD69 (HC273013) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of CD69 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow cytometry

Flow-cytometry using anti-Human CD69 antibody. Permeabilized Transfected cells and Control cells were stained with an anti-Human CD69 monoclonal antibody (Catalog HC273013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

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Anti-Human CD69 Recombinant Antibody (SRR0102).pdf

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Anti-Human CD69 Recombinant Antibody (SRR0102) (HC273013)

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