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| カタログ番号 | HB941063 |
|---|---|
| 説明 |
Anti-Human VEGF Recombinant Antibody (SRR0032) (HB941063) is a rabbit monoclonal antibody detecting VEGF in FCM. Suitable for Human.
Highlights
|
| 種反応性 | Human |
| アプリケーション | FCM |
| 宿主種 | Rabbit |
| クローン性 | Monoclonal |
| アイソタイプ | IgG |
| クローンID | SRR0032 |
| テスト済みアプリケーション | FCM |
| ターゲット | L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor |
| エンドトキシンレベル | < 10 EU/mg as determined by LAL method. |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P15692 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 背景 | Vascular endothelial growth factor A, long form (Vascular endothelial growth factor A,VPF,VEGFA,VEGF,Vascular permeability factor,VEGF-A) is a ~43 kDa protein. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. 1. Katsman, M. et al. (2022) Cells 11. PMID: 35455969 |
| 注意事項 | For research use only. |
Flow-cytometry using anti-Human VEGFA antibody. Permeabilized HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human VEGFA monoclonal antibody (Catalog HB941063, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-Human VEGFA antibody. Permeabilized U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human VEGFA monoclonal antibody (Catalog HB941063, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.
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