Anti-Human alpha-Fetoprotein/AFP-L3 Recombinant Antibody (SRR0473) (HY276043)

Western blot analysis was performed using anti-alpha-Fetoprotein/AFP-L3 monoclonal antibody at 1 ug/mL on various samples.
Lane 1: HepG2 cell lysate

HepG2 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting alpha-Fetoprotein/AFP-L3 (HY276043) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of alpha-Fetoprotein/AFP-L3 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Flow-cytometry using anti-Human AFP/AFP-L3 antibody. Permeabilized HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human AFP/AFP-L3 monoclonal antibody (Catalog HY276043 , Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.