Anti-CD284/TLR4 Polyclonal Antibody (HT205014)

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概要
イメージ
参考文献
データシート

概要

カタログ番号

HT205014

説明

Anti-CD284/TLR4 Polyclonal Antibody (HT205014) is a rabbit polyclonal antibody detecting CD284 in ELISA, IHC, WB. Suitable for Human, Pan paniscus, Papio anubis, and Pongo pygmaeus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Gorilla gorilla gorilla, Pan paniscus, Papio anubis, Pongo pygmaeus

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

アイソタイプ

IgG

クローン性

Polyclonal

免疫原

E. coli - derived recombinant Human CD284/TLR4 (Glu24-Lys631).

ターゲット

Toll-like receptor 4, TLR4, CD284, hToll

精製

Purified by antigen affinity column.

アクセッション番号

O00206

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Toll-like receptor 4 (CD284/TLR4) is a ~95 kDa protein. Transmembrane receptor that functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. At the plasma membrane, cooperates with LY96 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Mechanistically, acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Alternatively, CD14-mediated TLR4 internalization via endocytosis is associated with the initiation of a MYD88-independent signaling via the TICAM1-TBK1-IRF3 axis leading to type I interferon production.

1. Arbour, NC. et al. (2000) Nature genetics 25, 187-91. PMID: 10835634

2. Bulut, Y. et al. (2005) The Journal of biological chemistry 280, 20961-7. PMID: 15809303

3. Jung, SB. et al. (2006) Infection and immunity 74, 2686-96. PMID: 16622205

4. Georgel, P. et al. (2007) Virology 362, 304-13. PMID: 17292937

5. Kim, F. et al. (2007) Circulation research 100, 1589-96. PMID: 17478729

6. Stewart, CR. et al. (2010) Nature immunology 11, 155-61. PMID: 20037584

7. Schmidt, M. et al. (2010) Nature immunology 11, 814-9. PMID: 20711192

8. Estruch, M. et al. (2013) Atherosclerosis 229, 356-62. PMID: 23880187

9. Tatematsu, M. et al. (2016) Journal of immunology (Baltimore, Md. : 1950) 196, 3865-76. PMID: 27022195

10. Xiahou, Z. et al. (2017) Nature communications 8, 950. PMID: 29038465

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-CD284/TLR4 polyclonal antibody at 1ug/mL on recombinant CD284/TLR4 (Catalog No. HT205012).

参考文献

データシート