Anti-PPIA Polyclonal Antibody (HX128014)

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50ug
100ug

数量:

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概要
イメージ
参考文献
データシート

概要

カタログ番号

HX128014

説明

Anti-PPIA Polyclonal Antibody (HX128014) is a rabbit polyclonal antibody detecting PPIA in ELISA, IHC, WB. Suitable for Human, Aotus trivirgatus, Bovine, Cricetulus griseus, and Cat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Aotus trivirgatus, Bos taurus, Chlorocebus aethiops, Cricetulus griseus, Cat, Gorilla gorilla gorilla, Hylobates lar, Macaca mulatta, Mouse, Nomascus leucogenys, Oryctolagus cuniculus, Pan paniscus, Pan troglodytes, Papio anubis, Pongo pygmaeus, Rat, Saguinus oedipus, Pig, Symphalangus syndactylus

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

アイソタイプ

IgG

クローン性

Polyclonal

免疫原

E. coli - derived recombinant Human PPIA (Val2-Glu165).

ターゲット

PPIase A, Cyclosporin A-binding protein, Peptidyl-prolyl cis-trans isomerase A, Cyclophilin A, PPIA, CYPA, Rotamase A

精製

Purified by antigen affinity column.

アクセッション番号

P62937

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Peptidyl-prolyl cis-trans isomerase A (PPIA) is a ~18 kDa protein. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation. Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1. Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis. In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2.

1. Liu, J. et al. (1991) Biochemistry 30, 2306-10. PMID: 2001362

2. Davis, TL. et al. (2010) PLoS biology 8, e1000439. PMID: 20676357

3. Song, F. et al. (2011) The Journal of biological chemistry 286, 8197-8203. PMID: 21245143

4. Foster, TL. et al. (2011) Journal of virology 85, 7460-4. PMID: 21593166

5. Lauranzano, E. et al. (2015) Brain : a journal of neurology 138, 974-91. PMID: 25678563

6. Yurchenko, V. et al. (2002) The Journal of biological chemistry 277, 22959-65. PMID: 11943775

7. Jin, ZG. et al. (2004) Arteriosclerosis, thrombosis, and vascular biology 24, 1186-91. PMID: 15130913

8. Xie, Y. et al. (2019) Cellular signalling 61, 57-65. PMID: 31063815

9. Wei, Y. et al. (2013) Inflammation 36, 567-72. PMID: 23180369

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-PPIA polyclonal antibody at 1ug/mL on recombinant PPIA (Catalog No. HX128012).

参考文献

データシート

Anti-PPIA Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-PPIA Polyclonal Antibody (HX128014)

概要

イメージ

参考文献

データシート

クイックアクセス

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