Anti-Human Siglec-3/CD33 Recombinant Antibody (M195), APC-Cy7 (HB185257)

Name: Anti-Human Siglec-3/CD33 Recombinant Antibody (M195), APC-Cy7
Brand: abinScience
SKU: HB185257
Price: 435 USD
Availability: InStock

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Каталожный номер	HB185257
Описание	Anti-Human Siglec-3/CD33 Recombinant Antibody (M195), APC-Cy7 (HB185257) is a human monoclonal antibody detecting Siglec-3/CD33 in FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●APC-Cy7 Conjugated — Ready for direct detection without secondary antibody.
Реактивность видов	Human
Применения	FCM
Хозяинский вид	Human
Клоональность	Monoclonal
Изотип	IgG1, kappa
ID клона	M195
Конъюгация	APC-Cy7
Цель	Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, CD33, gp67, SIGLEC3
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P20138
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4, 0.2% BSA, 0.05% Proclin 300. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон	Myeloid cell surface antigen CD33 is a ~39 kDa protein. Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. CD33 is the therapeutic target of gemtuzumab ozogamicin (Mylotarg). 1. Vitale, C. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 15091-6. PMID: 10611343 2. Vitale, C. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 5764-9. PMID: 11320212 3. Lajaunias, F. et al. (2005) European journal of immunology 35, 243-51. PMID: 15597323 4. Freeman, SD. et al. (1995) Blood 85, 2005-12. PMID: 7718872 5. Paul, SP. et al. (2000) Blood 96, 483-90. PMID: 10887109 6. Son, M. et al. (2017) Scientific reports 7, 270. PMID: 28325905 7. Taylor, VC. et al. (1999) The Journal of biological chemistry 274, 11505-12. PMID: 10206955 8. Ulyanova, T. et al. (1999) European journal of immunology 29, 3440-9. PMID: 10556798
Примечание	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Anti-Human Siglec-3/CD33 Recombinant Antibody (M195), APC-Cy7.pdf

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Anti-Human Siglec-3/CD33 Recombinant Antibody (M195), APC-Cy7 (HB185257)

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