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| カタログ番号 | MY057407 |
|---|---|
| 説明 |
Anti-Mouse CD3E Antibody (145-2C11) [145-2C11] (MY057407) is a hamster monoclonal antibody detecting CD3e in FCM, ICC, IHC, IP, T cell stimulation (Reported), T cell depletion (Reported), WB. Suitable for Mouse.
Highlights
|
| 種反応性 | Mouse |
| アプリケーション | FCM, ICC, IHC, IP, T cell stimulation (Reported), T cell depletion (Reported), WB |
| 宿主種 | Hamster |
| アイソタイプ | IgG |
| クローンID | 145-2C11 |
| クローン性 | Monoclonal |
| ターゲット | T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P22646 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS, pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 背景 | T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~21 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto). 1. DeJarnette, JB. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 14909-14. PMID: 9843989 2. Saint-Ruf, C. et al. (2000) Nature 406, 524-7. PMID: 10952314 3. Wang, Y. et al. (2009) PLoS biology 7, e1000253. PMID: 19956738 4. Borroto, A. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 192, 2042-53. PMID: 24470497 5. Bettini, ML. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 193, 258-67. PMID: 24899501 6. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325 |
| 注意事項 | For research use only. |
SEC-HPLC detection for Anti-Mouse CD3E Antibody (145-2C11).
SDS-PAGE for Anti-Mouse CD3E Antibody (145-2C11)
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