Anti-Human IL12B/IL-12 p40/NKSF2 Antibody (SAA0381) (HB769107)

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概要
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データシート

概要

カタログ番号	HB769107
説明	Anti-Human IL12B/IL-12 p40/NKSF2 Antibody (SAA0381) [SAA0381] (HB769107) is a mouse monoclonal antibody detecting IL-12 subunit p40 in ELISA, FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
種反応性	Human
アプリケーション	ELISA, FCM
宿主種	Mouse
アイソタイプ	IgG1, lambda
クローンID	SAA0381
クローン性	Monoclonal
ターゲット	IL-12 subunit p40, IL12B, Interleukin-12 subunit beta, IL-12B, CLMF p40, NKSF2, NK cell stimulatory factor chain 2, Cytotoxic lymphocyte maturation factor 40 kDa subunit
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P29460
形態	Liquid
保存バッファー	0.01M PBS, pH 7.4, 0.09% Sodium azide. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Interleukin-12 subunit beta (IL12B/IL-12 p40) is a ~37 kDa protein. Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
注意事項	For research use only.

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Bioactivity

Detects Human IL12B/IL-12 p40/NKSF2 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human IL12B antibody.IL12B Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL12B antibody monoclonal antibody (Catalog # HB769107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.