ホーム > 製品 > 抗体 > フローサイトメトリー抗体

Anti-Human CD5 Antibody (5D7), FITC (HY046217)

データシート製品共有情報コピー

Anti-Human CD5 Antibody (5D7), FITC

Anti-Human CD5 Antibody (5D7), FITC

価格(USD): $

スペック:

50T
100T

数量:

お問い合わせ

概要
イメージ
参考文献
データシート

概要

カタログ番号	HY046217
説明	Anti-Human CD5 Antibody (5D7), FITC [5D7] (HY046217) is a mouse monoclonal antibody detecting CD5 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
種反応性	Human
アプリケーション	FCM
宿主種	Mouse
アイソタイプ	IgG1
クローンID	5D7
抱合	FITC
クローン性	Monoclonal
ターゲット	Lymphocyte antigen T1/Leu-1, CD5, T-cell surface glycoprotein CD5, LEU1
エンドトキシンレベル	Please contact with the lab for this information.
アクセッション番号	P06127
形態	Liquid
保存バッファー	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	T-cell surface glycoprotein CD5 is a ~54 kDa protein. Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. 1. Burgess, KE. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 9311-5. PMID: 1384049 2. Osman, N. et al. (1992) European journal of immunology 22, 2995-3000. PMID: 1385158 3. Roa, NS. et al. (2013) Biochemical and biophysical research communications 432, 52-9. PMID: 23376399 4. Garaud, S. et al. (2018) Cellular & molecular immunology 15, 158-170. PMID: 27499044
注意事項	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

イメージ

Flow Cytometry

Flow-cytometry using FITC anti-human CD5 antibody. Jurkat cells were stained with an irrelevant antibody (Green Histogram) or a FITC anti-human CD5 antibody monoclonal antibody (Catalog # HY046217, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献

データシート

Anti-Human CD5 Antibody (5D7), FITC.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

=

Concentration

×

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

×

V₁ (Stock Vol.)

=

Working Solution

C₂ (Working Conc.)

×

V₂ (Working Vol.)

レコメンデーション

お問い合わせカスタムクォート、大量リクエストおよびその他の問題については

メール: support@abinScience.com

ディストリビューターリスト

Anti-Human CD5 Antibody (5D7), FITC