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Anti-Mouse CD279/PDCD1/PD1 Antibody (29F.1A12), FITC (MS870217)

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概要

カタログ番号	MS870217
説明	Anti-Mouse CD279/PDCD1/PD1 Antibody (29F.1A12), FITC [29F.1A12] (MS870217) is a rat monoclonal antibody detecting PDCD1 in FCM, IF, WB. Suitable for Mouse. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
種反応性	Mouse
アプリケーション	FCM, IF, WB
宿主種	Rat
アイソタイプ	IgG2a, kappa
クローンID	29F.1A12
抱合	FITC
クローン性	Monoclonal
ターゲット	Programmed cell death protein 1, Protein PD-1, hPD-1, PD1, PDCD1, CD279
エンドトキシンレベル	Please contact with the lab for this information.
アクセッション番号	Q02242
形態	Liquid
保存バッファー	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	Programmed cell death protein 1 (CD279/PD-1) is a ~31 kDa protein. Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival. CD279 is the therapeutic target of pembrolizumab (Keytruda) and nivolumab (Opdivo). 1. Nishimura, H. et al. (1999) Immunity 11, 141-51. PMID: 10485649 2. Nishimura, H. et al. (2001) Science (New York, N.Y.) 291, 319-22. PMID: 11209085 3. Okazaki, T. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 13866-71. PMID: 11698646 4. Okazaki, T. et al. (2011) The Journal of experimental medicine 208, 395-407. PMID: 21300912 5. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443 6. Latchman, Y. et al. (2001) Nature immunology 2, 261-8. PMID: 11224527 7. Lin, DY. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 3011-6. PMID: 18287011 8. Lázár-Molnár, E. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 10483-8. PMID: 18641123 9. Yokosuka, T. et al. (2012) The Journal of experimental medicine 209, 1201-17. PMID: 22641383
注意事項	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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データシート

Anti-Mouse CD279/PDCD1/PD1 Antibody (29F.1A12), FITC.pdf

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Anti-Mouse CD279/PDCD1/PD1 Antibody (29F.1A12), FITC