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Anti-Human CD3 Antibody (UCHT1), PE (HY057627)

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概要
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データシート

概要

カタログ番号	HY057627
説明	Anti-Human CD3 Antibody (UCHT1), PE [UCHT1] (HY057627) is a human monoclonal antibody detecting T-cell surface glycoprotein CD3 epsilon chain in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
種反応性	Human
アプリケーション	FCM
宿主種	Human
アイソタイプ	IgG1, kappa
クローンID	UCHT1
抱合	PE
クローン性	Monoclonal
ターゲット	T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain
エンドトキシンレベル	Please contact with the lab for this information.
アクセッション番号	P07766
形態	Liquid
保存バッファー	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto). 1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938 2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002 3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098 4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325 5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660
注意事項	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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参考文献

データシート

Anti-Human CD3 Antibody (UCHT1), PE.pdf

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Anti-Human CD3 Antibody (UCHT1), PE