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Anti-Human CD20/MS4A1 Antibody (SAA2209), FITC (HY257617)

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Anti-Human CD20/MS4A1 Antibody (SAA2209), FITC

Anti-Human CD20/MS4A1 Antibody (SAA2209), FITC

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参考文献
データシート

概要

カタログ番号	HY257617
説明	Anti-Human CD20/MS4A1 Antibody (SAA2209), FITC [SAA2209] (HY257617) is a human monoclonal antibody detecting MS4A1 in ELISA, FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
種反応性	Human
アプリケーション	ELISA, FCM
宿主種	Human
アイソタイプ	IgG1, kappa
クローンID	SAA2209
抱合	FITC
クローン性	Monoclonal
ターゲット	B-lymphocyte surface antigen B1, Membrane-spanning 4-domains subfamily A member 1, MS4A1, Leukocyte surface antigen Leu-16, B-lymphocyte antigen CD20, Bp35, CD20
エンドトキシンレベル	Please contact with the lab for this information.
精製	Affinity chromatography.
アクセッション番号	P11836
形態	Liquid
保存バッファー	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	B-lymphocyte antigen CD20 (MS4A1) is a ~33 kDa protein. B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. CD20 is the therapeutic target of rituximab (MabThera) and obinutuzumab (Gazyva). 1. Li, H. et al. (2003) The Journal of biological chemistry 278, 42427-34. PMID: 12920111 2. Tedder, TF. et al. (1985) Journal of immunology (Baltimore, Md. : 1950) 135, 973-9. PMID: 3925015 3. Bubien, JK. et al. (1993) The Journal of cell biology 121, 1121-32. PMID: 7684739 4. Polyak, MJ. et al. (2008) The Journal of biological chemistry 283, 18545-52. PMID: 18474602
注意事項	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using FITC anti-Human CD20 antibody. Daudi cells were stained with an irrelevant antibody (Red Histogram) or a FITC anti-Human CD20 antibody monoclonal antibody (Catalog # HY257617 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献

データシート

Anti-Human CD20/MS4A1 Antibody (SAA2209), FITC.pdf

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Anti-Human CD20/MS4A1 Antibody (SAA2209), FITC