Anti-Human CD227/MUC1 Antibody (SAA2216) (HB102407)

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概要
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データシート

概要

カタログ番号	HB102407
説明	Anti-Human CD227/MUC1 Antibody (SAA2216) [SAA2216] (HB102407) is a human monoclonal antibody detecting CD227 in ELISA, FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
種反応性	Human
アプリケーション	ELISA, FCM
宿主種	Human
アイソタイプ	IgG1, kappa
クローンID	SAA2216
クローン性	Monoclonal
ターゲット	PEMT, CA 15-3, H23AG, Peanut-reactive urinary mucin, PEM, EMA, Cancer antigen 15-3, MUC-1, Episialin, MUC1-CT, MUC1-beta, Tumor-associated mucin, CD227, Polymorphic epithelial mucin, KL-6, MUC1-alpha, MUC1, PUM, Krebs von den Lungen-6, MUC1-NT, Tumor-associated epithelial membrane antigen, Mucin-1, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P15941
形態	Liquid
保存バッファー	0.01M PBS, pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Mucin-1 (CD227/MUC1) is a ~122 kDa protein. The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
注意事項	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human CD227 antibody. MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD227 antibody monoclonal antibody (Catalog # HB102407 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD227 antibody. CD227 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD227 antibody monoclonal antibody (Catalog # HB102407 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.