Research Grade Anti-Human CD66e/CEA/CEACAM5 (SGM 101) (HY546106)

データシート製品共有情報コピー

価格(USD): $

スペック:

100ug
1mg

数量:

お問い合わせ

概要
イメージ
参考文献
データシート

概要

カタログ番号	HY546106
説明	Anti-Human CD66e/CEA/CEACAM5 (SGM 101) (HY546106) is a research-grade recombinant antibody targeting CD66. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Human
アイソタイプ	IgG1-kappa
クローンID	SGM 101
発現システム	Mammalian Cells
クローン性	Monoclonal
ターゲット	Meconium antigen 100, CEACAM5, CEA, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen
エンドトキシンレベル	Please contact the lab for this information.
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P06731
形態	Liquid
保存バッファー	0.01M PBS, pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
別名	SGM-101, SGM101, SGM101
背景	Cell adhesion molecule CEACAM5 (CD66e) is a ~76 kDa protein. Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells. 1. Taheri, M. et al. (2000) The Journal of biological chemistry 275, 26935-43. PMID: 10864933 2. Ordoñez, C. et al. (2000) Cancer research 60, 3419-24. PMID: 10910050 3. Oikawa, S. et al. (1989) Biochemical and biophysical research communications 164, 39-45. PMID: 2803308
注意事項	For research use only. Not suitable for clinical or therapeutic use.

イメージ

Bioactivity

Detects recombinant human CD66e/CEA/CEACAM5 (Cat No.: HY546011) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD66e/CEA/CEACAM5 (SGM 101).
Western blot

Western blot analysis was performed using anti-CD66e/CEA/CEACAM5 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD66e/CEA/CEACAM5 (Catalog No: HY546011)
Lane 2: negative control
Flow cytometry

Flow-cytometry using PE anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD66e monoclonal antibody (Catalog HY546106, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD66e monoclonal antibody (Catalog HY546106, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.