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| カタログ番号 | VK432012 |
|---|---|
| 説明 |
Recombinant Nipah virus/NiV Protein N/Nucleoprotein Protein, N-His (VK432012) expressed in E. coli, spanning Thr32-Val532. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9IK92 |
| タンパク質長 | Thr32-Val532 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Nipah virus (NiV) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 57.14 kDa |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS, pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Nucleoprotein, Protein N, Nucleocapsid protein, N |
| 背景 | Nucleoprotein (Protein N) is a ~58 kDa protein. Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. The nucleocapsid (NC) has a helical structure with either 13.4 N per turn, approximately 20 nm in diameter, with a hollow central cavity of 56 nm in diameter. The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P. 1. Ker, DS. et al. (2021) PLoS pathogens 17, e1009740. PMID: 34270629 2. Yabukarski, F. et al. (2014) Nature structural & molecular biology 21, 754-9. PMID: 25108352 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Nipah virus/NiV Protein N/Nucleoprotein Protein, N-His
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