中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HP036022 |
|---|---|
| 説明 |
Recombinant Human ZMYND8 Protein, N-His (HP036022) expressed in E. coli, spanning Val80-Asn400. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9ULU4 |
| タンパク質長 | Val80-Asn400 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 39.49 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Protein kinase C-binding protein 1, PRKCBP1, RACK7, ZMYND8, CTCL-associated antigen se14-3, Cutaneous T-cell lymphoma-associated antigen se14-3, Rack7, KIAA1125, Zinc finger MYND domain-containing protein 8 |
| 背景 | MYND-type zinc finger-containing chromatin reader ZMYND8 is a ~131 kDa protein. Chromatin reader that recognizes dual histone modifications such as histone H3.1 dimethylated at 'Lys-36' and histone H4 acetylated at 'Lys-16' (H3.1K36me2-H4K16ac) and histone H3 methylated at 'Lys-4' and histone H4 acetylated at 'Lys-14' (H3K4me1-H3K14ac). May act as a transcriptional corepressor for KDM5D by recognizing the dual histone signature H3K4me1-H3K14ac. May also act as a transcriptional corepressor for KDM5C and EZH2. Recognizes acetylated histone H4 and recruits the NuRD chromatin remodeling complex to damaged chromatin for transcriptional repression and double-strand break repair by homologous recombination. Also activates transcription elongation by RNA polymerase II through recruiting the P-TEFb complex to target promoters. 1. Adhikary, S. et al. (2016) The Journal of biological chemistry 291, 2664-81. PMID: 26655721 2. Li, N. et al. (2016) Molecular cell 63, 470-84. PMID: 27477906 3. Mukherjee, S. et al. (2020) Journal of biosciences 45. PMID: 31965980 4. Adhikary, S. et al. (2022) Cell death & disease 13, 766. PMID: 36064715 5. Mukherjee, S. et al. (2020) Cell death & disease 11, 1073. PMID: 33323928 6. Gong, F. et al. (2015) Genes & development 29, 197-211. PMID: 25593309 7. Spruijt, CG. et al. (2016) Cell reports 17, 783-798. PMID: 27732854 8. Ghosh, K. et al. (2018) Cell reports 24, 2141-2154.e6. PMID: 30134174 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human ZMYND8 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
