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Mouse AIFM1 Recombinant Protein (N-His) (MF658012)

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Aperçu
Numéro de catalogueMF658012
Description
Recombinant Mouse AIFM1 Protein, N-His (MF658012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsQ9Z0X1
Longueur de protéineVal131-Lys321
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesMus musculus (Mouse)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsAif, Aifm1, Apoptosis-inducing factor 1, mitochondrial, EC:1.6.99.-, Pdcd8, Programmed cell death protein 8
Contexte

Apoptosis-inducing factor 1, mitochondrial is a ~66 kDa protein. Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA.

1. Yu, SW. et al. (2002) Science (New York, N.Y.) 297, 259-63. PMID: 12114629
2. Wang, Y. et al. (2011) Science signaling 4, ra20. PMID: 21467298
3. Susin, SA. et al. (1999) Nature 397, 441-6. PMID: 9989411
4. Sevrioukova, IF. (2009) Journal of molecular biology 390, 924-38. PMID: 19447115
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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C₂ (Working Conc.)
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