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| カタログ番号 | MV826012 |
|---|---|
| 説明 |
Recombinant Mouse PYCARD Protein, N-His (MV826012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9EPB4 |
| タンパク質長 | Met1-Ser193 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Mus musculus (Mouse) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Apoptosis-associated speck-like protein containing a CARD, Asc, PYD and CARD domain-containing protein, Pycard, mASC |
| 背景 | Apoptosis-associated speck-like protein containing a CARD is a ~21 kDa protein. Functions as a key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in innate immune response by acting as an integral adapter in the assembly of various inflammasomes (NLRP2, NLRP3, NLRP6 and AIM2) which recruit and activate caspase-1 leading to processing and secretion of pro-inflammatory cytokines. Caspase-1-dependent inflammation leads to macrophage pyroptosis, a form of cell death. 1. Mukherjee, S. et al. (2020) Nature immunology 21, 626-635. PMID: 32424362 2. Shen, C. et al. (2021) Cell 184, 5759-5774.e20. PMID: 34678144 3. Mariathasan, S. et al. (2004) Nature 430, 213-8. PMID: 15190255 4. Yamamoto, M. et al. (2004) Genes to cells : devoted to molecular & cellular mechanisms 9, 1055-67. PMID: 15507117 5. Ippagunta, SK. et al. (2011) Nature immunology 12, 1010-6. PMID: 21892172 6. Pierini, R. et al. (2012) Cell death and differentiation 19, 1709-21. PMID: 22555457 7. Wang, Y. et al. (2017) Immunity 46, 393-404. PMID: 28314590 |
| 注意事項 | For research use only |
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