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| カタログ番号 | JN179032 |
|---|---|
| 説明 |
Recombinant M. tuberculosis Amidase Rv3717 Protein, N-His (JN179032) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | I6Y4D2 |
| タンパク質長 | Ala37-Arg253 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Mycobacterium tuberculosis |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | EC:3.5.1.28, N-acetylmuramoyl-L-alanine amidase Rv3717, Zinc-dependent peptidoglycan amidase |
| 背景 | N-acetylmuramoyl-L-alanine amidase Rv3717 is a ~24 kDa protein. Cell-wall hydrolase that hydrolyzes the amide bond between N-acetylmuramic acid and L-alanine in cell-wall glycopeptides. Is able to hydrolyze the cell walls of several bacterial species (i.e. Paenibacillus sp., B.avium, E.coli DH5alpha, E.aerogenes, L.acidophilus, B.thuringiensis, B.pumilus, B.subtilis and E.coli W3110), thereby showing that it is a cell-wall hydrolase with broad-spectrum activity. May have a role in peptidoglycan fragment recycling. 1. Prigozhin, DM. et al. (2013) The Journal of biological chemistry 288, 31549-55. PMID: 24019530 2. Kumar, A. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 2543-54. PMID: 24311595 |
| 注意事項 | For research use only |
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